This study was aimed to evaluate the psychometric properties and the relationship of age with the answers, internal consistency and factorial structure of the TCI-R. The questionnaire was responded by a sample of 928 voluntary subjects (396 males and 532 women), with a mean age of 30 years old. The participants under 30 scored substantially higher in Novelty Seeking, and to a lesser extent in Reward Dependence, while aged subjects scored significant higher in Harm Avoidance, Self-Directiveness and Cooperation. The global coefficients of factorial congruence were higher than 0.90 in all age groups. The goodness-of-fit indexes in the CFA were unsatisfactory for the simple structure models, although improved when considering secondary loadings. Implications for further research with the TCI-R are discussed.